Anti-TDP43 antibody (ab41881)
Key features and details
- Rabbit polyclonal to TDP43
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TDP43 antibody
See all TDP43 primary antibodies -
Description
Rabbit polyclonal to TDP43 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TARDBP.
Read Abcam's proprietary immunogen policy (Peptide available as ab41970.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).Target
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Function
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. -
Tissue specificity
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. -
Involvement in disease
Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The RRM domains can bind to both DNA and RNA. -
Post-translational
modificationsHyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. -
Cellular localization
Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies. - Information by UniProt
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Database links
- Entrez Gene: 23435 Human
- Entrez Gene: 230908 Mouse
- Omim: 605078 Human
- SwissProt: Q13148 Human
- SwissProt: Q921F2 Mouse
- Unigene: 300624 Human
- Unigene: 635053 Human
- Unigene: 22453 Mouse
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Alternative names
- ALS10 antibody
- OTTHUMP00000002171 antibody
- OTTHUMP00000002172 antibody
see all
Images
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Western blot - Anti-TDP43 antibody (ab41881)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: TDP43 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: Jurkat cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41881 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.ab41881 was shown to specifically react with TDP43 when TDP43 knockout samples were used. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE. Ab41881 and ab8245 (loading control to GAPDH) were diluted at 1 µg/mL and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-TDP43 antibody (ab41881)All lanes : Anti-TDP43 antibody (ab41881) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 :Hep G2 nuclear extract lysate (ab14660)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : K-562 (Human) Nuclear Lysate - tumor cell line (ab29309)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab41881 has been referenced in 7 publications.
- Neelagandan N et al. TDP-43 enhances translation of specific mRNAs linked to neurodegenerative disease. Nucleic Acids Res 47:341-361 (2019). PubMed: 30357366
- Li P et al. A liver-enriched long non-coding RNA, lncLSTR, regulates systemic lipid metabolism in mice. Cell Metab 21:455-67 (2015). PubMed: 25738460
- Huang CC et al. Metabolism and mis-metabolism of the neuropathological signature protein TDP-43. J Cell Sci 127:3024-38 (2014). PubMed: 24860144
- Dang TN et al. Endogenous progesterone levels and frontotemporal dementia: modulation of TDP-43 and Tau levels in vitro and treatment of the A315T TARDBP mouse model. Dis Model Mech 6:1198-204 (2013). WB . PubMed: 23798570
- Braun RJ et al. Neurotoxic 43-kDa TAR DNA-binding Protein (TDP-43) Triggers Mitochondrion-dependent Programmed Cell Death in Yeast. J Biol Chem 286:19958-72 (2011). WB ; Saccharomyces cerevisiae . PubMed: 21471218
- Shan X et al. Altered distributions of Gemini of coiled bodies and mitochondria in motor neurons of TDP-43 transgenic mice. Proc Natl Acad Sci U S A 107:16325-30 (2010). IHC-FoFr ; Mouse . PubMed: 20736350
- Chen WQ et al. Variations of protein levels in human amniotic fluid stem cells CD117/2 over passages 5-25. J Proteome Res 8:5285-95 (2009). PubMed: 19791749
Images
-
Western blot - Anti-TDP43 antibody (ab41881)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: TDP43 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: Jurkat cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41881 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.ab41881 was shown to specifically react with TDP43 when TDP43 knockout samples were used. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE. Ab41881 and ab8245 (loading control to GAPDH) were diluted at 1 µg/mL and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-TDP43 antibody (ab41881)All lanes : Anti-TDP43 antibody (ab41881) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 :Hep G2 nuclear extract lysate (ab14660)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : K-562 (Human) Nuclear Lysate - tumor cell line (ab29309)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
