Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23745-311] to TDO2/TDO
Suitable for: ICC, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-TDO2/TDO antibody [EPR23745-311]
See all TDO2/TDO primary antibodies
Description

Rabbit monoclonal [EPR23745-311] to TDO2/TDO
Host species

Rabbit
Tested applications

Suitable for: ICC, IP, WBmore details
Unsuitable for: Flow Cyt (Intra),IHC-Fr or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human liver, Mouse liver, Rat liver lysates. ICC: 293T cells. IP: Human liver, Mouse liver lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23745-311
Isotype

IgG

Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

All lanes : Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution

Lane 1 : Human liver tissue lysate
Lane 2 : Human pancreas tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 48 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The the expression pattern and observed MW is consistent with what has been described in the literature (PMID: 27573671, PMID: 25691885)

Negative control: pancreas (PMID:25691885).

Exposure time: 26 seconds
Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

All lanes : Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse pancreas tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat pancreas tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 48 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression pattern and observed MW is consistent with what has been described in the literature (PMID:25691885, PMID: 10775460).

Negative control: pancreas (PMID:25691885).

Exposure time: 26 seconds.
Immunocytochemistry - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling TDO2/TDO with ab259359 at 1/500 (1.072 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Green) Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged TDO2 expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.
Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

TDO2/TDO was immunoprecipitated from 0.35 mg Human liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human liver tissue lysate 10 ug

Lane 2: ab259359 IP in Human liver tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259359 in human liver tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

TDO2/TDO was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse liver tissue lysate 10 ug

Lane 2: ab259359 IP in Mouse liver tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259359 in mouse liver tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

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