Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)
![Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)](https://www.abcam.com/ps/products/259/ab259359/Images/ab259359-458941-anti-tdo2tdo-antibody-epr23745311-western-blot-lysate.jpg "Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23745-311] to TDO2/TDO
- Suitable for: ICC, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TDO2/TDO antibody [EPR23745-311]
See all TDO2/TDO primary antibodies -
Description
Rabbit monoclonal [EPR23745-311] to TDO2/TDO -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details
Unsuitable for: Flow Cyt (Intra),IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver, Mouse liver, Rat liver lysates. ICC: 293T cells. IP: Human liver, Mouse liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23745-311 -
Isotype
IgG
Images
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Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)All lanes : Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human pancreas tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The the expression pattern and observed MW is consistent with what has been described in the literature (PMID: 27573671, PMID: 25691885)
Negative control: pancreas (PMID:25691885).
Exposure time: 26 seconds
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![Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)](https://www.abcam.com/ps/products/259/ab259359/Images/ab259359-458943-anti-tdo2tdo-antibody-epr23745311-western-blot-lysate1.jpg)
Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)All lanes : Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse pancreas tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat pancreas tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern and observed MW is consistent with what has been described in the literature (PMID:25691885, PMID: 10775460).
Negative control: pancreas (PMID:25691885).
Exposure time: 26 seconds.
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![Immunocytochemistry - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)](https://www.abcam.com/ps/products/259/ab259359/Images/ab259359-1-anti-tdo2tdo-antibody-epr23745311-immunocytochemistry-293t.jpg)
Immunocytochemistry - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling TDO2/TDO with ab259359 at 1/500 (1.072 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Green) Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged TDO2 expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.
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![Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)](https://www.abcam.com/ps/products/259/ab259359/Images/ab259359-2-anti-tdo2tdo-antibody-epr23745311-immunoprecipitation-human-liver-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)TDO2/TDO was immunoprecipitated from 0.35 mg Human liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human liver tissue lysate 10 ug
Lane 2: ab259359 IP in Human liver tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259359 in human liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)](https://www.abcam.com/ps/products/259/ab259359/Images/ab259359-3-anti-tdo2tdo-antibody-epr23745311-immunoprecipitation-mouse-liver-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)TDO2/TDO was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate 10 ug
Lane 2: ab259359 IP in Mouse liver tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259359 in mouse liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)](https://www.abcam.com/ps/products/259/ab259359/Images/ab259359-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)