Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP613Y] to Rel B - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Rel B antibody [EP613Y] - BSA and Azide free
    See all Rel B primary antibodies

  • Description

    Rabbit monoclonal [EP613Y] to Rel B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Raji whole cell lysate.

  • General notes

    Ab247280 is the carrier-free version of ab33917. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247280 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    Western blot - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    All lanes : Anti-Rel B antibody [EP613Y] (ab33917) at 1/500 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP labeled

    Predicted band size: 62 kDa



    This data was developed using ab33917, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    This data was developed using ab33917, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lymphoma using ab33917 Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    Flow Cytometry - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    This data was developed using ab33917, the same antibody clone in a different buffer formulation.Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Rel B (red) with ab33917 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    Immunoprecipitation - Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)

    This data was developed using ab33917, the same antibody clone in a different buffer formulation.
    Purified ab33917 at 1/50 dilution (2µg) immunoprecipitating Rel B in Raji whole cell lysate.
    Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab33917 + Raji whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33917 in Raji whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 62 kDa

  • Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)
    Anti-Rel B antibody [EP613Y] - BSA and Azide free (ab247280)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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