Anti-TCR V beta 5.3 antibody [W112] (ab171099)
![Anti-TCR V beta 5.3 antibody [W112] (ab171099)](https://www.abcam.com/ps/products/171/ab171099/Images/ab171099-215955-anti-tcr-v-beta-53-antibody-w112-flow-cytometry.jpg "Anti-TCR V beta 5.3 antibody [W112] (ab171099)")
Key features and details
- Mouse monoclonal [W112] to TCR V beta 5.3
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-TCR V beta 5.3 antibody [W112] -
Description
Mouse monoclonal [W112] to TCR V beta 5.3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length protein corresponding to Human TCR V beta 5.3.
Database link: P04435 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.5% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
W112 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-TCR V beta 5.3 antibody [W112] (ab171099)
Flow cytometry analysis of TCR V beta 5b in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V beta 5b monoclonal antibody (ab171099) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
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![Flow Cytometry - Anti-TCR V beta 5.3 antibody [W112] (ab171099)](https://www.abcam.com/ps/products/171/ab171099/Images/ab171099-215954-anti-tcr-v-beta-53-antibody-w112-flow-cytometry.jpg)
Flow Cytometry - Anti-TCR V beta 5.3 antibody [W112] (ab171099)Flow cytometry analysis of TCR V beta 5b in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab171099) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.