Anti-TCHP antibody (ab77622)
Key features and details
- Rabbit polyclonal to TCHP
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCHP antibody -
Description
Rabbit polyclonal to TCHP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide corresponding to Human TCHP aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab91616) -
Positive control
- This antibody gave a positive signal in human tonsil tissue lysate and in the whole cell lysate: U87MG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab77622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa). Target
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Relevance
A tumour suppressor protein which may act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. -
Cellular localization
Cytoplasmic, cytoskeleton -
Database links
- Entrez Gene: 84260 Human
- Omim: 612654 Human
- SwissProt: Q9BT92 Human
- Unigene: 410924 Human
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Alternative names
- TCHP antibody
- Trichoplein keratin filament-binding protein antibody
- Tumor suppressor protein antibody
Images
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Western blot - Anti-TCHP antibody (ab77622)All lanes : Anti-TCHP antibody (ab77622) at 1 µg/ml
Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-TCHP antibody (ab77622)All lanes : Anti-TCHP antibody (ab77622) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human skin tissue lysate - total protein (ab30166)
Lane 4 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 5 :T47D whole cell lysate (ab14899)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-TCHP antibody (ab77622)ICC/IF image of ab77622 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77622, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCHP antibody (ab77622)IHC image of TCHP staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 77622, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Datasheets and documents
References (0)
ab77622 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TCHP antibody (ab77622)All lanes : Anti-TCHP antibody (ab77622) at 1 µg/ml
Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
-
Western blot - Anti-TCHP antibody (ab77622)All lanes : Anti-TCHP antibody (ab77622) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human skin tissue lysate - total protein (ab30166)
Lane 4 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 5 :T47D whole cell lysate (ab14899)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-TCHP antibody (ab77622)ICC/IF image of ab77622 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77622, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCHP antibody (ab77622)IHC image of TCHP staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 77622, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.