Anti-TC1 antibody (ab229680)
Key features and details
- Rabbit polyclonal to TC1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TC1 antibody -
Description
Rabbit polyclonal to TC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant fragment within Human TC1 (internal sequence). The exact sequence is proprietary.
Database link: O60779 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse liver tissue extract. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TC1 antibody (ab229680)All lanes : Anti-TC1 antibody (ab229680) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa10% SDS-PAGE gel.
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Western blot - Anti-TC1 antibody (ab229680)Anti-TC1 antibody (ab229680) at 1/1000 dilution + Mouse liver tissue extract at 50 µg
Predicted band size: 55 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-TC1 antibody (ab229680)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TC1 (green) using ab229680 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
