Anti-Tau (phospho T181) antibody (ab75679)
Key features and details
- Rabbit polyclonal to Tau (phospho T181)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Tau (phospho T181) antibody
See all Tau primary antibodies -
Description
Rabbit polyclonal to Tau (phospho T181) -
Host species
Rabbit -
Specificity
ab75679 detects endogenous levels of isoform Tau-F, which is a truncated form of the unprocessed precursor isoform PNS-tau (http://www.uniprot.org/uniprot/P10636)), where the phosphorylation site is located at threonine 181. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Tau. (PKT(phospho)PP)
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Positive control
- Rat hippocampal region tissue from a model with Alzheimer’s Disease and extracts from mouse brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75679 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody (ab75679)ab75679 at 1/50 dilution staining Tau in rat hippocampal region tissue from a model with Alzheimer’s Disease by Immunohistochemistry, Paraffin-embedded tissue.
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Western blot - Anti-Tau (phospho T181) antibody (ab75679)All lanes : Anti-Tau (phospho T181) antibody (ab75679) at 1/500 dilution
Lane 1 : mouse brain tissue extracts with immunising peptide
Lane 2 : mouse brain tissue extracts
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
