Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)
![Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)](https://www.abcam.com/ps/products/92/ab92627/Images/ab92627-107807-anti-tau-phospho-s262-t263-antibody-epr2454-western-blot.jpg "Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2454] to Tau (phospho S262 + T263)
- Suitable for: Dot blot, ELISA, WB
- Reacts with: Human
Overview
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Product name
Anti-Tau (phospho S262 + T263) antibody [EPR2454]
See all Tau primary antibodies -
Description
Rabbit monoclonal [EPR2454] to Tau (phospho S262 + T263) -
Host species
Rabbit -
Specificity
WB band is consistent with what is found in PMID 16682510 Peng et. al -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Tau aa 250-350 (phospho S262 + T263). The exact sequence is proprietary.
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Positive control
- SH-SY5Y cell lysate treated with olcadaic acid and Calyculin A
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2454 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)All lanes : Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627) at 1/2000 dilution
Lane 1 : SH-SY5Y cell lysate treated with Olcadaic acid & Calyculin A
Lane 2 : SH-SY5Y cell lysate untreated
Predicted band size: 79 kDa
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![ELISA - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)](https://www.abcam.com/ps/products/92/ab92627/Images/ab92627-284189-anti-tau-phospho-s262-t263-antibody-epr2454-elisa.jpg)
ELISA - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)Serially diluted ab92627 was bound to immobilised phospho S262, phospho T263 or non phospho Tau peptide (100 ng/mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97051; diluted at 1:100,000) and signal was developed with ECL substrate.
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![Dot Blot - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)](https://www.abcam.com/ps/products/92/ab92627/Images/ab92627-284183-anti-tau-phospho-s262-t263-antibody-epr2454-dot-blot.jpg)
Dot Blot - Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)Dot blot analysis of Tau (pS262) peptide (Lane 1), Tau (pT263) peptide (Lane 2) and Tau non-phospho peptide (Lane 3) labelling Tau (pS262 + pT263) with ab92627 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100,000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)](https://www.abcam.com/ps/products/92/ab92627/Images/ab92627-4-benefits-of-recombinant-antibodies.png)
Anti-Tau (phospho S262 + T263) antibody [EPR2454] (ab92627)
