Antibodies, Proteins, Kits and Reagents for Life Science

241 228 ₸ Product size

100 µl 241 228 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Tau antibody [EPR2396(2)] (ab109392)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2396(2)] to Tau
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Tau antibody [EPR2396(2)]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR2396(2)] to Tau
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human brain tissue; human glioma tissue. ICC/IF: SHSY5Y cells. WB: Human Hippocampus whole cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2396(2)
Isotype

IgG
Research areas

Western blot - Anti-Tau antibody [EPR2396(2)] (ab109392)

Anti-Tau antibody [EPR2396(2)] (ab109392) at 1/1000 dilution + Human Hippocampus whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 50-70 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR2396(2)] (ab109392)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109392.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR2396(2)] (ab109392)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue using 1/250 ab109392.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EPR2396(2)] (ab109392)

Immunofluorescent staining of SHSY5Y cells using 1/100 ab109392.
Anti-Tau antibody [EPR2396(2)] (ab109392)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com