Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976)
![Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976)](https://www.abcam.com/ps/products/185/ab185976/Images/ab185976-218076-anti-tap-tag-antibody-22f12-1e3-c-terminal-western-blot.jpg "Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976)")
Key features and details
- Mouse monoclonal [22F12-1E3] to TAP tag - C-terminal
- Suitable for: WB
- Isotype: IgG1
Overview
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Product name
Anti-TAP tag antibody [22F12-1E3] - C-terminal -
Description
Mouse monoclonal [22F12-1E3] to TAP tag - C-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Synthetic peptide corresponding to TAP tag (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage (Tobacco Etch Virus)
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Positive control
- TAP-tag containing control protein.
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General notes
TAP adds about 20 kDa to the size of the protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.1% BSA, 69% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
22F12-1E3 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976)All lanes : Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976) at 1/1000 dilution
Lane 1 : TAP-tag containing control protein at 20 µl
Lane 2 : TAP-tag containing control protein at 10 µl
Lane 3 : TAP-tag containing control protein at 5 µl
Secondary
All lanes : goat anti-mouse IgG-HRP at 1/15000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
