Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15433] to TAF5 - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TAF5 antibody [EPR15433] - BSA and Azide free
See all TAF5 primary antibodies -
Description
Rabbit monoclonal [EPR15433] to TAF5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251201 is the carrier-free version of ab195236.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR15433 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab195236, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab195236 at 1/70 dilution (lane 1) or a Rabbit monoclonal IgG (lane 2).
Western Blot performed using ab195236 at 1/1000 dilution (0.6µg/ml).
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
This data was developed using ab195236, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling TAF5 with ab195236 at 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
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All lanes : Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 86 kDaThis data was developed using ab195236, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID: 9045704).
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All lanes : Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 86 kDaThis data was developed using ab195236, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID: 9045704).
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