Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7850] to IP10 - BSA and Azide free
  • Suitable for: ELISA, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IP10 antibody [EPR7850] - BSA and Azide free
    See all IP10 primary antibodies

  • Description

    Rabbit monoclonal [EPR7850] to IP10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml) for 32 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate; IP10 recombinant protein.

  • General notes

    ab248769 is the carrier-free version of ab137018 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248769 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)
    Western blot - Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)
    All lanes : Anti-IP10 antibody [EPR7850] (ab137018) at 1/500 dilution

    Lane 1 : Wild-type A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate
    Lane 2 : Wild-type A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml) for 32 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate
    Lane 3 : IP10 knockout A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate
    Lane 4 : IP10 knockout A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml) for 32 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 10 kDa
    Observed band size: 11 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab137018).

    Lanes 1 - 4: Merged signal (red and green). Green - ab137018 observed at 11 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab137018 was shown to react with IP10 in A549 wild-type cells in western blot with loss of signal observed in IP10 knockout cell line ab266969 (IP10 knockout cell lysate ab256886). A549 wild-type and IP10 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab137018 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)
    Western blot - Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)
    Anti-IP10 antibody [EPR7850] (ab137018) at 1/1000 dilution + IP10 recombinant protein at 0.01 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 10 kDa



    This data was developed using ab137018, the same antibody clone in a different buffer formulation.

     

  • ELISA - Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)
    ELISA - Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)

    This data was developed using ab137018, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human IP10 recombinant protein at 1000 ng/mL with ab137018. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)
    Anti-IP10 antibody [EPR7850] - BSA and Azide free (ab248769)

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