Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15433] to TAF5 - C-terminal
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TAF5 antibody [EPR15433] - C-terminal
    See all TAF5 primary antibodies

  • Description

    Rabbit monoclonal [EPR15433] to TAF5 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, MCF7, HepG2, C6 and RAW 264.7 and 293 cell lysates; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15433

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    Immunoprecipitation - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)

    Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab195236 at 1/70 dilution (lane 1) or a Rabbit monoclonal IgG (lane 2).
    Western Blot performed using ab195236 at 1/1000 dilution (0.6µg/ml).
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Flow Cytometry - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    Flow Cytometry - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)

    Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling TAF5 with ab195236 at 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

  • Western blot - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    Western blot - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    All lanes : Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 86 kDa



    The observed MW is consistent with what has been described in the literature (PMID:9045704)

  • Western blot - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    Western blot - Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    All lanes : Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 86 kDa



    The observed MW is consistent with what has been described in the literature (PMID:9045704)

  • Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)
    Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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