Anti-Syndecan 2/HSPG antibody (ab205884)
Key features and details
- Rabbit polyclonal to Syndecan 2/HSPG
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Syndecan 2/HSPG antibody -
Description
Rabbit polyclonal to Syndecan 2/HSPG -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Syndecan 2/HSPG (internal sequence).
Database link: P34741 -
Positive control
- A549, HepG2, HT-20, K562, TF-1, U2OS and DU 145 cell lysates; Caco cells; Human hepatocellular carcinoma tissue; HeLa cells.
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General notes
Protein previously labeled as Syndecan 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Syndecan 2/HSPG antibody (ab205884)
Flow Cytometrical analysis of ethanol fixed permeabilized HeLa cells labeling Syndecan 2/HSPG with ab205884 at 1/100 (red) compared to a rabbit isotype control (pink histogram). The cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1/400. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
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Western blot - Anti-Syndecan 2/HSPG antibody (ab205884)All lanes : Anti-Syndecan 2/HSPG antibody (ab205884) at 2 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HT-20 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : TF-1 cell lysate
Lane 6 : U2OS cell lysate
Predicted band size: 22 kDa
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Western blot - Anti-Syndecan 2/HSPG antibody (ab205884)All lanes : Anti-Syndecan 2/HSPG antibody (ab205884) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : DU 145 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22,27 kDa why is the actual band size different from the predicted?12 % Bis-Tris gel
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan 2/HSPG antibody (ab205884)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Syndecan 2/HSPG with ab205884 at 1/100. Image on the left shows a negative control without primary antibody. Detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-Syndecan 2/HSPG antibody (ab205884)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Syndecan 2/HSPG with ab205884 at 2 µg/ml and Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control.