All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution

Lane 1 : BK virus infected 293TT whole cell lysate
Lane 2 : Mock infected 293TT whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?


Exposure time: 4 minutes

See Abreview

SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3

ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.

ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.