Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
![Anti-KSHV ORF45 antibody [2D4A5] (ab36618)](https://www.abcam.com/ps/products/36/ab36618/Images/ab36618_WB.gif "Anti-KSHV ORF45 antibody [2D4A5] (ab36618)")
Key features and details
- Mouse monoclonal [2D4A5] to KSHV ORF45
- Suitable for: WB, ICC, ELISA
- Reacts with: Human herpesvirus 8
- Isotype: IgG1
Overview
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Product name
Anti-KSHV ORF45 antibody [2D4A5] -
Description
Mouse monoclonal [2D4A5] to KSHV ORF45 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, ELISAmore details -
Species reactivity
Reacts with: Human herpesvirus 8 -
Immunogen
Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3).
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Positive control
- TPA induced BCBL-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2D4A5 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-KSHV ORF45 antibody [2D4A5] (ab36618)All lanes : Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
Lane 1 : Molecular weight marker
Lane 2 : Recombinant KSHV ORF45 expressed in E.coli
Predicted band size: 50 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Based on the analysis of the mRNA sequence, ORF45 is expected to encode a protein of 407 amino acids with a molecular mass of about 50 kDa. However, ORF45 was displayed as a diffuse band at the position of 78 kDa on a Western blot after SDS-PAGE. This observation implied that ORF45 might be highly post-translationally modified, resulting in its reduced mobility in denaturing SDS-PAGE. A survey of the primary amino acid sequence of ORF45 revealed multiple potential sites of phosphorylation, including a serine-rich region and other consensus sites for phosphorylation by the serine/threonine kinase, casein kinase II, and protein kinase C (Zhu & Yuan, 2003). -
![Immunocytochemistry - Anti-KSHV ORF45 antibody [2D4A5] (ab36618)](https://www.abcam.com/ps/products/36/ab36618/Images/ab36618_ICC.gif)
Immunocytochemistry - Anti-KSHV ORF45 antibody [2D4A5] (ab36618)Immunochemistry staining of TPA-induced BCBL-1 cells, using ab36618. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.