Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SV2C antibody (ab33892)

Key features and details

  • Rabbit polyclonal to SV2C
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-SV2C antibody

  • Description

    Rabbit polyclonal to SV2C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide corresponding to Rat SV2C aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab33891)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab33892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern; the highest expression levels being observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb.

    • Cellular localization

      Integral Membrane Protein

    • Database links

      see all

    • Alternative names

      • Synaptic vesicle protein 2c antibody

    Images

    • Western blot - Anti-SV2C antibody (ab33892)
      Western blot - Anti-SV2C antibody (ab33892)
      Anti-SV2C antibody (ab33892) at 1 µg/ml + Olfactory Bulb (Rat) Tissue Lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 82 kDa
      Observed band size: 82 kDa
      Additional bands at: 30 kDa (possible non-specific binding), 36 kDa (possible non-specific binding)


      Exposure time: 4 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33892 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.

    • Western blot - Anti-SV2C antibody (ab33892)
      Western blot - Anti-SV2C antibody (ab33892)
      All lanes : Anti-SV2C antibody (ab33892) at 1 µg/ml

      Lane 1 : Substantia Nigra (Mouse) Tissue Lysate
      Lane 2 : Olfactory Bulb (Mouse) Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 82 kDa
      Observed band size: 82 kDa
      Additional bands at: 30 kDa (possible non-specific binding), 36 kDa (possible non-specific binding)


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33892 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.

    • Immunocytochemistry/ Immunofluorescence - Anti-SV2C antibody (ab33892)
      Immunocytochemistry/ Immunofluorescence - Anti-SV2C antibody (ab33892)
      ICC/IF image of ab33892 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33892, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

    References (1)

    Publishing research using ab33892? Please let us know so that we can cite the reference in this datasheet.

    ab33892 has been referenced in 1 publication.

    • Couesnon A  et al. Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine. PLoS Pathog 8:e1002583 (2012). WB ; Mouse . PubMed: 22438808

    Images

    • Western blot - Anti-SV2C antibody (ab33892)
      Western blot - Anti-SV2C antibody (ab33892)
      Anti-SV2C antibody (ab33892) at 1 µg/ml + Olfactory Bulb (Rat) Tissue Lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 82 kDa
      Observed band size: 82 kDa
      Additional bands at: 30 kDa (possible non-specific binding), 36 kDa (possible non-specific binding)


      Exposure time: 4 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33892 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.

    • Western blot - Anti-SV2C antibody (ab33892)
      Western blot - Anti-SV2C antibody (ab33892)
      All lanes : Anti-SV2C antibody (ab33892) at 1 µg/ml

      Lane 1 : Substantia Nigra (Mouse) Tissue Lysate
      Lane 2 : Olfactory Bulb (Mouse) Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 82 kDa
      Observed band size: 82 kDa
      Additional bands at: 30 kDa (possible non-specific binding), 36 kDa (possible non-specific binding)


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33892 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.

    • Immunocytochemistry/ Immunofluorescence - Anti-SV2C antibody (ab33892)
      Immunocytochemistry/ Immunofluorescence - Anti-SV2C antibody (ab33892)
      ICC/IF image of ab33892 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33892, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

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