Anti-Sulfotyrosine antibody [7C5] (ab243069)
![Anti-Sulfotyrosine antibody [7C5] (ab243069)](https://www.abcam.com/ps/products/243/ab243069/Images/ab243069-332303-69b.jpg "Anti-Sulfotyrosine antibody [7C5] (ab243069)")
Key features and details
- Mouse monoclonal [7C5] to Sulfotyrosine
- Suitable for: IHC-P, WB
- Isotype: IgG1
Overview
-
Product name
Anti-Sulfotyrosine antibody [7C5]
See all Sulfotyrosine primary antibodies -
Description
Mouse monoclonal [7C5] to Sulfotyrosine -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Immunogen
Chemical/ Small Molecule corresponding to Sulfotyrosine conjugated to keyhole limpet haemocyanin.
-
Positive control
- WB: Sulfotyrosine-BSA. IHC-P: Human kidney tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
7C5 -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [7C5] (ab243069)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Sulfotyrosine using ab243069 at 1/25 dilution for 1 hour at room temperature in immunohistochemical analysis (C). The nuclear counterstain is DAPI (blue, A). Actin is detected with Actin-binding Phalloidin-AlexaFluor® 633 (red, B). (D) is a composite image.
Secondary Antibody: Goat Anti-Mouse IgG: AlexaFluor® 488.
-
![Western blot - Anti-Sulfotyrosine antibody [7C5] (ab243069)](https://www.abcam.com/ps/products/243/ab243069/Images/ab243069-332302-69a.jpg)
Western blot - Anti-Sulfotyrosine antibody [7C5] (ab243069)All lanes : Anti-Sulfotyrosine antibody [7C5] (ab243069) at 1/1000 dilution
Lane 1 : Sulfotyrosine-BSA
Lane 2 : Tyrosine-BSA
Lane 3 : Phosphotyrosine-BSA
Lane 4 : BSA
Lysates/proteins at 2 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Lane 4 : Goat Anti-Mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
