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Anti-Sulfotyrosine antibody [7C5] (ab243069)

Key features and details

  • Mouse monoclonal [7C5] to Sulfotyrosine
  • Suitable for: IHC-P, WB
  • Isotype: IgG1

Overview

  • Product name

    Anti-Sulfotyrosine antibody [7C5]
    See all Sulfotyrosine primary antibodies

  • Description

    Mouse monoclonal [7C5] to Sulfotyrosine

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Immunogen

    Chemical/ Small Molecule corresponding to Sulfotyrosine conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: Sulfotyrosine-BSA. IHC-P: Human kidney tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    7C5

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [7C5] (ab243069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfotyrosine antibody [7C5] (ab243069)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for Sulfotyrosine using ab243069 at 1/25 dilution for 1 hour at room temperature in immunohistochemical analysis (C). The nuclear counterstain is DAPI (blue, A). Actin is detected with Actin-binding Phalloidin-AlexaFluor® 633 (red, B). (D) is a composite image.

    Secondary Antibody: Goat Anti-Mouse IgG: AlexaFluor® 488.

  • Western blot - Anti-Sulfotyrosine antibody [7C5] (ab243069)
    Western blot - Anti-Sulfotyrosine antibody [7C5] (ab243069)
    All lanes : Anti-Sulfotyrosine antibody [7C5] (ab243069) at 1/1000 dilution

    Lane 1 : Sulfotyrosine-BSA
    Lane 2 : Tyrosine-BSA
    Lane 3 : Phosphotyrosine-BSA
    Lane 4 : BSA

    Lysates/proteins at 2 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
    Lane 4 : Goat Anti-Mouse IgG at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Primary incubation for 2 hours at room temperature.

    Secondary incubation for 1 hour at room temperature.

    Block: 5% Skimmed milk in TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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