Anti-SSB antibody [EPR6570] (ab124932)
![Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.com/ps/products/124/ab124932/Images/ab124932-138962-anti-ssb-antibody-epr6570-western-blot.jpg "Anti-SSB antibody [EPR6570] (ab124932)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6570] to SSB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SSB antibody [EPR6570]
See all SSB primary antibodies -
Description
Rabbit monoclonal [EPR6570] to SSB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human SSB aa 100-200. The exact sequence is proprietary.
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Positive control
- Jurkat, K562, HeLa, Ramos, C6, PC12, NIH 3T3 and RAW 264.7 cell lysates; Human colon tissue; Human thyroid adenocarcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6570 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SSB antibody [EPR6570] (ab124932)All lanes : Anti-SSB antibody [EPR6570] (ab124932) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : C6 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.com/ps/products/124/ab124932/Images/ab124932-138961-anti-ssb-antibody-epr6570-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)Formalin-fixed, paraffin-embedded human colon tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.com/ps/products/124/ab124932/Images/ab124932-138960-anti-ssb-antibody-epr6570-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)Formalin-fixed, paraffin-embedded human thyroid adenocarcinoma tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.com/ps/products/124/ab124932/Images/ab124932-138959-anti-ssb-antibody-epr6570.jpg)
OI-RD Scanning - Anti-SSB antibody [EPR6570] (ab124932) -
![Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.com/ps/products/124/ab124932/Images/ab124932-5-benefits-of-recombinant-antibodies.png)
Anti-SSB antibody [EPR6570] (ab124932)
