Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
![Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-397738-anti-sr-protein-repeatsrsf6-antibody-epr9231-western-blot-human-knockout-lysate.jpg "Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SR protein repeat/SRSF6 antibody [EPR9231]
See all SR protein repeat/SRSF6 primary antibodies -
Description
Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human SR protein repeat/SRSF6 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: HEK293T, K562, Jurkat and HeLa whole cell lysates. IHC-P: Human gastric, ovarian and brain carcinoma tissue; Human uterus and brain tissue.
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General notes
This product was previously labelled as SR protein repeat
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9231 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SRSF6 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab140623 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.
ab140623 Anti-SR protein repeat/SRSF6 antibody [EPR9231] was shown to specifically react with SR protein repeat/SRSF6 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266529 (knockout cell lysate ab258210) was used. Wild-type and SR protein repeat/SRSF6 knockout samples were subjected to SDS-PAGE. ab140623 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155916-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling SR protein repeat/SRSF6 with ab140623 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155917-anti-sr-protein-repeat-antibody-epr9231-western-blot.jpg)
Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155915-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155914-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155913-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155912-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-8-benefits-of-recombinant-antibodies.png)
Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
