Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBMY1A1 antibody [R12508(2)] (ab200202)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [R12508(2)] to RBMY1A1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RBMY1A1 antibody [R12508(2)]
    See all RBMY1A1 primary antibodies

  • Description

    Rabbit monoclonal [R12508(2)] to RBMY1A1

  • Host species

    Rabbit

  • Specificity

    Based on sequence analysis, ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis lysate. IHC-P: Human testis tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RBMY1A1 antibody [R12508(2)] (ab200202)
    Western blot - Anti-RBMY1A1 antibody [R12508(2)] (ab200202)
    All lanes : Anti-RBMY1A1 antibody [R12508(2)] (ab200202) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 51,56 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Please note lanes 2-5 represent RBMY1A1 negative lysates.

    Based on sequence analysis, ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] (ab200202)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] (ab200202)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RBMY1A1 with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining of Human testis tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] (ab200202)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] (ab200202)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin. 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Please note Human liver tissue represents a RBMY1A1 negative control.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-RBMY1A1 antibody [R12508(2)] (ab200202)
    Anti-RBMY1A1 antibody [R12508(2)] (ab200202)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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