Anti-Spastin antibody [Sp 3G11/1] (ab31850)
![Anti-Spastin antibody [Sp 3G11/1] (ab31850)](https://www.abcam.com/ps/products/31/ab31850/Images/ab31850-43130-anti-spastin-antibody-sp-3g11-1-western-blot.jpg "Anti-Spastin antibody [Sp 3G11/1] (ab31850)")
Key features and details
- Mouse monoclonal [Sp 3G11/1] to Spastin
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Spastin antibody [Sp 3G11/1]
See all Spastin primary antibodies -
Description
Mouse monoclonal [Sp 3G11/1] to Spastin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein (Human).
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Positive control
- WB: Total HeLa extract or rat brain synaptosome This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma. IP: HAP1 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Sp 3G11/1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Spastin antibody [Sp 3G11/1] (ab31850)Anti-Spastin antibody [Sp 3G11/1] (ab31850) at 1/500 dilution + rat brain synaptosome
Observed band size: 52 kDa why is the actual band size different from the predicted?
There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spastin antibody [Sp 3G11/1] (ab31850)](https://www.abcam.com/ps/products/31/ab31850/Images/ab31850-43129-anti-spastin-antibody-sp-3g11-1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spastin antibody [Sp 3G11/1] (ab31850)IHC image of Spastin staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31850, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
