Anti-MAP4 antibody (ab245578)
Key features and details
- Rabbit polyclonal to MAP4
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP4 antibody
See all MAP4 primary antibodies -
Description
Rabbit polyclonal to MAP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human MAP4 aa 1102-1152. The exact sequence is proprietary. NP_002366.2
Database link: P27816 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245578 was affinity purified using an epitope specific to MAP4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAP4 antibody (ab245578)All lanes : Anti-MAP4 antibody (ab245578) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 121 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-MAP4 antibody (ab245578)MAP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245578 used for IP at 3 µg/mg lysate. For WB 0.01 µg/ml.
Lane 1: ab245578 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab245578)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for MAP4 with ab245578 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
