Anti-MAP4 antibody (ab224543)
Key features and details
- Rabbit polyclonal to MAP4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP4 antibody
See all MAP4 primary antibodies -
Description
Rabbit polyclonal to MAP4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MAP4 aa 550-650.
Database link: P27816 -
Positive control
- WB: U-251 MG sp cell lysate; Human liver and tonsil lysates. IHC-P: Human heart muscle, skeletal muscle, and kidney tissue. ICC/IF: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human kidney stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin-embedded human soft tissue tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-MAP4 antibody (ab224543) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 121 kDa
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Paraffin-embedded human heart muscle tissue stained for MAP4 using ab224543 at 1/1000 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MAP4 (green) using ab224543 at 4 µg/ml in ICC/IF.