Anti-HOOK1 antibody (ab241429)
Key features and details
- Rabbit polyclonal to HOOK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HOOK1 antibody
See all HOOK1 primary antibodies -
Description
Rabbit polyclonal to HOOK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human HOOK1 aa 678-728. The exact sequence is proprietary.
Database link: Q9UJC3 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241429 was affinity purified using an epitope specific to HOOK1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-HOOK1 antibody (ab241429)All lanes : Anti-HOOK1 antibody (ab241429) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-HOOK1 antibody (ab241429)HOOK1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241429 used for IP at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241429 at 1 µg/ml.Lane 1: ab241429 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
