Anti-Spastin antibody [Sp 6C6] (ab77144)
![Anti-Spastin antibody [Sp 6C6] (ab77144)](https://www.abcam.com/ps/products/77/ab77144/Images/ab77144-410467-anti-spastin-antibody-sp-6c6-western-blot-human-knockout-lysate.jpg "Anti-Spastin antibody [Sp 6C6] (ab77144)")
Key features and details
- Mouse monoclonal [Sp 6C6] to Spastin
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Spastin antibody [Sp 6C6]
See all Spastin primary antibodies -
Description
Mouse monoclonal [Sp 6C6] to Spastin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein (Human)
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Positive control
- WB: HEK293T cell lysate; Rat brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Sp 6C6 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Spastin antibody [Sp 6C6] (ab77144)All lanes : Anti-Spastin antibody [Sp 6C6] (ab77144) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SPAST knockout HEK293T cell lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab77144 observed at 52 kDa. Red - loading control ab181602 observed at 36 kDa.
ab77144 Anti-Spastin antibody [Sp 6C6] was shown to specifically react with Spastin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267238 (knockout cell lysate ab258698) was used. Wild-type and Spastin knockout samples were subjected to SDS-PAGE. ab77144 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Spastin antibody [Sp 6C6] (ab77144)](https://www.abcam.com/ps/products/77/ab77144/Images/ab77144-228393-ab77144wb.jpg)
Western blot - Anti-Spastin antibody [Sp 6C6] (ab77144) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Spastin antibody [Sp 6C6] (ab77144) at 1/1500 dilution
All lanes : Human induced pluripotent stem cell derived neuron whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/10 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 54,58,62,68 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes9% SDS-PAGE.
