Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SPAM1 antibody [EPR22906-57] (ab231771)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22906-57] to SPAM1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SPAM1 antibody [EPR22906-57]
    See all SPAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22906-57] to SPAM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis tissue lysate. IHC-P: Human testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SPAM1 antibody [EPR22906-57] (ab231771)
    Western blot - Anti-SPAM1 antibody [EPR22906-57] (ab231771)
    All lanes : Anti-SPAM1 antibody [EPR22906-57] (ab231771) at 1/1000 dilution

    Lane 1 : Human testis tissue
    Lane 2 : Human spleen tissue
    Lane 3 : Human skeletal muscle tissue
    Lane 4 : Human brain tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 58 kDa
    Observed band size: 64 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The molecular weight  observed is consistent with what has been described in the literature (PMID: 12932297).

    Negative control: Human spleen, human skeletal muscle, human brain (PMID: 8575780, 16330764).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAM1 antibody [EPR22906-57] (ab231771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAM1 antibody [EPR22906-57] (ab231771)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SPAM1 with ab231771 at 1/250 dilution (2.11µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID/ 12932297) is observed. The section was incubated with ab231771 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Anti-SPAM1 antibody [EPR22906-57] (ab231771)
    Anti-SPAM1 antibody [EPR22906-57] (ab231771)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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