Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 16, 2021

Anti-SPAM1 antibody [EPR22906-57] (ab231771)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22906-57] to SPAM1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-SPAM1 antibody [EPR22906-57]
See all SPAM1 primary antibodies
Description

Rabbit monoclonal [EPR22906-57] to SPAM1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human testis tissue lysate. IHC-P: Human testis tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22906-57
Isotype

IgG
Research areas

Western blot - Anti-SPAM1 antibody [EPR22906-57] (ab231771)

All lanes : Anti-SPAM1 antibody [EPR22906-57] (ab231771) at 1/1000 dilution

Lane 1 : Human testis tissue
Lane 2 : Human spleen tissue
Lane 3 : Human skeletal muscle tissue
Lane 4 : Human brain tissue

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 58 kDa
Observed band size: 64 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID: 12932297).

Negative control: Human spleen, human skeletal muscle, human brain (PMID: 8575780, 16330764).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAM1 antibody [EPR22906-57] (ab231771)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SPAM1 with ab231771 at 1/250 dilution (2.11µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID/ 12932297) is observed. The section was incubated with ab231771 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-SPAM1 antibody [EPR22906-57] (ab231771)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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