Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [OTI3D3] to SOCS3
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human, Recombinant fragment
Isotype: IgG2a

Product name

Anti-SOCS3 antibody [OTI3D3]
See all SOCS3 primary antibodies
Description

Mouse monoclonal [OTI3D3] to SOCS3
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Recombinant fragment
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human SOCS3 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_003946).
Database link: O14543

Run BLAST with Run BLAST with
Positive control
- WB: pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate. IHC-P: Human colon adenocarcinoma and thyroid carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA. Flow: HeLa and Jurkat cells.
General notes

Clone OTI3D3 (formerly 3D3).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI3D3
Isotype

IgG2a
Research areas

Western blot - Anti-SOCS3 antibody [OTI3D3] (ab236519)

All lanes : Anti-SOCS3 antibody [OTI3D3] (ab236519) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (ab236519)

Paraffin-embedded human colon adenocarcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (ab236519)

Paraffin-embedded human thyroid carcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] (ab236519)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA stained for SOCS3 (green) using ab236519 at 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] (ab236519)

Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).
Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] (ab236519)

Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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