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Anti-SOCS3 antibody [OTI3D3] (ab236519)

Key features and details

  • Mouse monoclonal [OTI3D3] to SOCS3
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

Overview

  • Product name

    Anti-SOCS3 antibody [OTI3D3]
    See all SOCS3 primary antibodies

  • Description

    Mouse monoclonal [OTI3D3] to SOCS3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Recombinant fragment
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human SOCS3 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_003946).
    Database link: O14543

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate. IHC-P: Human colon adenocarcinoma and thyroid carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA. Flow: HeLa and Jurkat cells.

  • General notes

    Clone OTI3D3 (formerly 3D3).

Properties

Images

  • Western blot - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    Western blot - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    All lanes : Anti-SOCS3 antibody [OTI3D3] (ab236519) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (ab236519)

    Paraffin-embedded human colon adenocarcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] (ab236519)

    Paraffin-embedded human thyroid carcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] (ab236519)

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA stained for SOCS3 (green) using ab236519 at 1/100 dilution in ICC/IF.

  • Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] (ab236519)

    Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

  • Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] (ab236519)
    Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] (ab236519)

    Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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