Anti-CASC5/KNL1 antibody (ab70537)
Key features and details
- Rabbit polyclonal to CASC5/KNL1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CASC5/KNL1 antibody
See all CASC5/KNL1 primary antibodies -
Description
Rabbit polyclonal to CASC5/KNL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CASC5/KNL1.
Database link: NP_733468.2 -
Positive control
- Whole cell lysate from HeLa or 293T cells
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General notes
This product was previously labelled as CASC5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CASC5/KNL1 antibody (ab70537) at 1/5000 dilution
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Developed using the ECL technique.
Predicted band size: 265 kDa
Observed band size: >300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70537 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot, 20% of the precipitate was loaded per lane, and ab70537 was used at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.