Anti-LAP3 antibody [EPR10330] (ab154809)
![Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-397739-anti-lap3-antibody-epr10330-western-blot-human-knockout-lysate.jpg "Anti-LAP3 antibody [EPR10330] (ab154809)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10330] to LAP3
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-LAP3 antibody [EPR10330]
See all LAP3 primary antibodies -
Description
Rabbit monoclonal [EPR10330] to LAP3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
(Peptide available asab200873) -
Positive control
- WB: A549, 293T, HepG2 and HeLa cell lysates; Human fetal liver lysate. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt: 293T cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10330 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : LAP3 knockout A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaLanes 1-4: Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266986 (knockout cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161352-anti-lap3-antibody-epr10330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161350-anti-lap3-antibody-epr10330-flow-cytometry.jpg)
Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)Flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green). -
![Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161353-anti-lap3-antibody-epr10330-western-blot.jpg)
Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161351-anti-lap3-antibody-epr10330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161349-anti-lap3-antibody-epr10330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161348-anti-lap3-antibody-epr10330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161347-anti-lap3-antibody-epr10330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-161346-anti-lap3-antibody-epr10330-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-LAP3 antibody [EPR10330] (ab154809)](https://www.abcam.com/ps/products/154/ab154809/Images/ab154809-10-benefits-of-recombinant-antibodies.png)
Anti-LAP3 antibody [EPR10330] (ab154809)
