Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-LAP3 antibody [EPR10330] (ab154809)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10330] to LAP3
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-LAP3 antibody [EPR10330]
See all LAP3 primary antibodies
Description

Rabbit monoclonal [EPR10330] to LAP3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
(Peptide available as ab200873)
Positive control
- WB: A549, 293T, HepG2 and HeLa cell lysates; Human fetal liver lysate. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt: 293T cells.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10330
Isotype

IgG
Research areas

Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : LAP3 knockout A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Lanes 1-4: Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.

ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266986 (knockout cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)

Flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-LAP3 antibody [EPR10330] (ab154809)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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