Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8539] to SNAP23 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SNAP23 antibody [EPR8539] - BSA and Azide free
    See all SNAP23 primary antibodies

  • Description

    Rabbit monoclonal [EPR8539] to SNAP23 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248624 is the carrier-free version of ab133703. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248624 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)
    Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)

    This data was developed using ab133703, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: SNAP23 knockout HAP1 cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab133703 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133703 was shown to specifically react with SNAP23 when SNAP23 knockout samples were used. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab133703 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)
    Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)
    All lanes : Anti-SNAP23 antibody [EPR8539] (ab133703) at 1/1000 dilution

    Lane 1 : Fetal liver cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : BxPC-3 cell lysate

    Predicted band size: 23 kDa



    This data was developed using ab133703, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)
    OI-RD Scanning - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)
    This data was developed using ab133703, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)
    Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (ab248624)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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