Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8543] to Syntaxin 3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Syntaxin 3 antibody [EPR8543]
    See all Syntaxin 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8543] to Syntaxin 3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human Syntaxin 3 (Q13277).

  • Positive control

    • WB: HepG2, Caco-2, , 293T, BxPC-3, Raw 264.7, PC-12, Rat lung, and NIH/3T3 lysates. IHC-P: human kidney, mouse kidney, and rat kidney tissues. ICC/IF: PC-12 and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling Syntaxin 3 with Purified ab133750 at 1:50 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    All lanes : Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 4 : Rat lung lysates
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Syntaxin 3 with Purified ab133750 at 1:250 dilution (0.104 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Western blot - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    All lanes : Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : Raw 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH3T3 cell lysate
    Lane 7 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Syntaxin 3 with ab133750 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Immunocytochemical analysis of HepG2 cells labelling Syntaxin 3 with ab133750 at 1/50.
  • OI-RD Scanning - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    OI-RD Scanning - Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Syntaxin 3 antibody [EPR8543] (ab133750)
    Anti-Syntaxin 3 antibody [EPR8543] (ab133750)

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