Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

All lanes : Anti-Smoothelin antibody [R4A] (ab8969)

Lane 1 : uterine artery
Lane 2 : uterine corpus.

Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.

Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by ab8969) and actin (green fluorescence).

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.

Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.

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Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.