Anti-Smoothelin antibody (ab74379)
Key features and details
- Rabbit polyclonal to Smoothelin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smoothelin antibody
See all Smoothelin primary antibodies -
Description
Rabbit polyclonal to Smoothelin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Smoothelin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab74378) -
Positive control
- This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHorseCowDogPigOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).Target
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Function
Structural protein of the cytoskeleton. -
Tissue specificity
Smooth muscle; contractile or vascular (for the long form). -
Sequence similarities
Belongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain. -
Cellular localization
Cytoplasm > cytoskeleton. Exhibits a filamentous organization. - Information by UniProt
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Database links
- Entrez Gene: 6525 Human
- Entrez Gene: 29856 Mouse
- Entrez Gene: 289734 Rat
- Omim: 602127 Human
- SwissProt: P53814 Human
- SwissProt: Q921U8 Mouse
- Unigene: 149098 Human
- Unigene: 188516 Mouse
see all -
Alternative names
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
see all
Images
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Western blot - Anti-Smoothelin antibody (ab74379)All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100,95 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This antibody was raised against an immunogen that is predicted to cross react with all isoforms of Smoothelin, with exception to Isoform A (Short).
Datasheets and documents
References (0)
ab74379 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Smoothelin antibody (ab74379)All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100,95 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This antibody was raised against an immunogen that is predicted to cross react with all isoforms of Smoothelin, with exception to Isoform A (Short).
