Anti-Smoothelin antibody (ab204603)
Key features and details
- Rabbit polyclonal to Smoothelin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smoothelin antibody
See all Smoothelin primary antibodies -
Description
Rabbit polyclonal to Smoothelin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Smoothelin aa 400-550.
Database link: P53814 -
Positive control
- WB: RT-4 whole cell lysate; IHC-P: Human prostate and rectum tissue;
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody (ab204603)
Immunohistochemical analysis of human prostate tissue labeling Smoothelin in the cytoplasm of smooth muscle cells with ab204603 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-Smoothelin antibody (ab204603)Anti-Smoothelin antibody (ab204603) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysate
Predicted band size: 99 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody (ab204603)Immunohistochemical analysis of human rectum tissue labeling Smoothelin in the cytoplasm of smooth muscle cells with ab204603 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody (ab204603)Immunohistochemical analysis of human lymph node tissue labeling Smoothelin with ab204603 at a 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody (ab204603)Immunohistochemical analysis of human liver tissue labeling Smoothelin with ab204603 at a 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
