Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smoothelin antibody [EPR20044] (ab219652)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20044] to Smoothelin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Smoothelin antibody [EPR20044]
    See all Smoothelin primary antibodies

  • Description

    Rabbit monoclonal [EPR20044] to Smoothelin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human bladder and uterus lysates; Mouse and rat colon lysates. IHC-P: Human colon, stomach, skeletal muscle, bladder cancer and gastrointestinal stromal tumor (GIST) tissues; Mouse colon and heart tissues; Rat colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on visceral smooth muscle of human stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle of human bladder cancer. Negative staining on tumor cells. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on scattered tumor cells of human gastrointestinal stromal tumor (PMID: 19950405). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle of skeletal muscle. Negative staining on skeletal muscle cells. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of mouse colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cardiac vascular smooth muscle cells.  Negative staining on myocardium. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of rat colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. 

    Negative control: No staining on rat skeletal muscle (PMID: 8707825). 

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652)
    All lanes : Anti-Smoothelin antibody [EPR20044] (ab219652) at 1/1000 dilution

    Lane 1 : Human bladder lysate at 20 µg
    Lane 2 : Human uterus lysate at 10 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/4000 dilution
    Lane 2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110,59 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 3 seconds.

    This antibody reacts with the 59 kDa and 110 kDa bands, corresponding to isoforms A and B respectively. Smoothelin is exclusively found in smooth muscle cells (PMID:8707825).

  • Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652)
    Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652)
    All lanes : Anti-Smoothelin antibody [EPR20044] (ab219652) at 1/1000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat colon lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 99 kDa
    Observed band size: 59 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2: 2 seconds.

    This antibody reacts with a 59 kDa band in mouse and rat colon smooth muscle.

  • Anti-Smoothelin antibody [EPR20044] (ab219652)
    Anti-Smoothelin antibody [EPR20044] (ab219652)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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