Anti-SMC1A (phospho S957) antibody (ab240573)
Key features and details
- Rabbit polyclonal to SMC1A (phospho S957)
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SMC1A (phospho S957) antibody
See all SMC1A primary antibodies -
Description
Rabbit polyclonal to SMC1A (phospho S957) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide within Human SMC1A (phospho S957). The exact sequence is proprietary. (NP_006297.2).
Database link: Q14683 -
Positive control
- WB: Etoposide treated HEK-293T whole cell lysate. IP: Etoposide treated HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue; Mouse teratoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab240573 was affinity purified using an epitope specific to SMC1A (phospho S957) immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC1A (phospho S957) antibody (ab240573)All lanes : Anti-SMC1A (phospho S957) antibody (ab240573) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (Mock treated) whole cell lysate
Lane 2 : HEK-293T, Treated with 100 uM etoposide for 2 hours, whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody (ab240573)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for SMC1A (phospho S957) with ab240573 at 1 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody (ab240573)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for (phospho S957) with ab240573 at 0.2 µg/ml in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-SMC1A (phospho S957) antibody (ab240573)Detection of SMC1A (phospho S957) by IP.
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) treated with 100 µM etopside for 2 hours (Lane 2) or mock treated (Lane 1).
ab240573 used at 6 µg/mg lysate and at 0.1 µg/ml for WB.
Lane 3: Control IgG IP in HEK-293T whole cell lysate mock treated.
Lane 4: Control IgG IP in HEK-293T whole cell lysate treated with 100 µM etopside for 2 hours.
Detection: Chemiluminescence with exposure times of 30 seconds.
