Anti-SMC1A antibody (ab9262)
Key features and details
- Rabbit polyclonal to SMC1A
- Suitable for: IHC-P, ICC, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SMC1A antibody
See all SMC1A primary antibodies -
Description
Rabbit polyclonal to SMC1A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human SMC1A aa 1133-1233 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q14683 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC1A antibody (ab9262)All lanes : Anti-SMC1A antibody (ab9262) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 143 kDa
Exposure time: 3 secondsDetection: Chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SMC1A with ab9262 at 1/1000 (1µg/ml). Detection: DAB.
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Immunocytochemistry - Anti-SMC1A antibody (ab9262) This image is courtesy of Kirk McManus, University of British ColumbiaInterphase HeLa cells stained with ab9262 (1/500). The antibody gave nuclear staining in all interphase nuclei investigated. However, the antibody failed to recognize any chromatin-associated epitopes in prophase or metaphase cells suggesting that the epitopes may be masked during mitosis. ab9262 staining is shown in green. The cells were counterstained with DAPI (red). 100x magnification.
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Immunoprecipitation - Anti-SMC1A antibody (ab9262)Immunoprecipitation analysis of HeLa whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lanes 1 & 2: ab9262 was used for IP at 6 µg per reaction.
Lane 3: IP using rabbit anti-SMC1A antibody (ab140493).
Lane 4: Control IgG.For western blotting immunoprecipitated SMC1, ab9262 was used at 1 µg/ml.
Detection: Chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)Ab9262 staining human normal colon tissue. Staining is localised to nuclear compartment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
