Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465) - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free
    See all SMAD5 primary antibodies

  • Description

    Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467).

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247460 is the carrier-free version of ab76296 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247460 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)
    Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)
    All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution

    Lane 1 : HeLa cell lysate - untreated
    Lane 2 : HeLa cell lysate - treated with BMP-4

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    This data was developed using ab76296, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)
    Immunocytochemistry - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)
    This data was developed using ab76296, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution.
  • Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)
    Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - BSA and Azide free (ab247460)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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