Anti-Smad2 antibody [5G7] (ab119907)
Key features and details
- Mouse monoclonal [5G7] to Smad2
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Smad2 antibody [5G7]
See all Smad2 primary antibodies -
Description
Mouse monoclonal [5G7] to Smad2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF HumanIHC-P HumanWB Human -
Immunogen
Purified recombinant fragment of Human Smad2 expressed in E. Coli.
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Positive control
- Recombinant protein; Smad2-hIgGFc transfected HEK293 cell lysate; Human liver cancer and cerebellum tissues; U251 and NIH3T3 cells.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR163661-5 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5G7 -
Isotype
IgG1 -
Research areas
Images
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Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution + Recombinant Human Smad2 protein
Predicted band size: 52 kDa
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All lanes : Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate.
Lane 2 : Smad2-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 52 kDa
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ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human liver cancer tissues by Immunohistochemistry followed by DAB staining.
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ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry followed by DAB staining.
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ab119907 at 1/200 dilution staining Smad2 in U251 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA showing use of ab119907.
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ab119907 at 1/200 dilution staining Smad2 in NIH3T3 cells by Flow Cytometry (blue) and negative control (red).