Anti-Smad2 antibody [5G7] (ab119907)
![Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133376-anti-smad2-antibody-5g7-western-blot.jpg "Anti-Smad2 antibody [5G7] (ab119907)")
Key features and details
- Mouse monoclonal [5G7] to Smad2
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Smad2 antibody [5G7]
See all Smad2 primary antibodies -
Description
Mouse monoclonal [5G7] to Smad2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Purified recombinant fragment of Human Smad2 expressed in E. Coli.
-
Positive control
- Recombinant protein; Smad2-hIgGFc transfected HEK293 cell lysate; Human liver cancer and cerebellum tissues; U251 and NIH3T3 cells.
-
General notes
This product was changed from ascites to supernatant. Lot no’s high than GR163661-5 are from Tissue Culture Supernatant
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5G7 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-Smad2 antibody [5G7] (ab119907)Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution + Recombinant Human Smad2 protein
Predicted band size: 52 kDa
-
![Western blot - Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133375-anti-smad2-antibody-5g7-western-blot.jpg)
Western blot - Anti-Smad2 antibody [5G7] (ab119907)All lanes : Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate.
Lane 2 : Smad2-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 52 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133374-anti-smad2-antibody-5g7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody [5G7] (ab119907)ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human liver cancer tissues by Immunohistochemistry followed by DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133373-anti-smad2-antibody-5g7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody [5G7] (ab119907)ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry followed by DAB staining. -
![Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133372-anti-smad2-antibody-5g7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody [5G7] (ab119907)ab119907 at 1/200 dilution staining Smad2 in U251 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![ELISA - Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133371-anti-smad2-antibody-5g7-elisa.jpg)
ELISA - Anti-Smad2 antibody [5G7] (ab119907)ELISA showing use of ab119907. -
![Flow Cytometry - Anti-Smad2 antibody [5G7] (ab119907)](https://www.abcam.com/ps/products/119/ab119907/Images/ab119907-133370-anti-smad2-antibody-5g7-flow-cytometry.jpg)
Flow Cytometry - Anti-Smad2 antibody [5G7] (ab119907)ab119907 at 1/200 dilution staining Smad2 in NIH3T3 cells by Flow Cytometry (blue) and negative control (red).
