Anti-Smad1 antibody (ab63356)
Key features and details
- Rabbit polyclonal to Smad1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad1 antibody
See all Smad1 primary antibodies -
Description
Rabbit polyclonal to Smad1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic non-phosphopeptide derived from human Smad1 around the phosphorylation site of serine 187.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad1 antibody (ab63356)All lanes : Anti-Smad1 antibody (ab63356) at 1/500 dilution
Lane 1 : extracts from 293 cell line
Lane 2 : extracts from 293 cell line, with immunizing peptide at 5 µg
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug. -
Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody (ab63356)Immunofluorescence analysis of Smad1 expression in HeLa cells, using 1/500 ab63356 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 antibody (ab63356)ab63356 (1µg/ml) staining SMAD1 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
