Anti-SHC3/SHCC antibody (ab233477)
Key features and details
- Rabbit polyclonal to SHC3/SHCC
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SHC3/SHCC antibody -
Description
Rabbit polyclonal to SHC3/SHCC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human SHC3/SHCC aa 150-450. N-terminal His-tag and GST-tag; Expressed in E.coli.
Database link: Q92529 -
Positive control
- WB: Pig brain lysate; HepG2 cell lysate; Recombinant human SHC3/SHCC protein. IHC-P: Human liver, glioma, kidney, stomach cancer, breast cancer and cerebrum tissue.
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General notes
This product was previously labelled as SHC3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (ab233477)
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SHC3/SHCC using ab233477 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (ab233477)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SHC3/SHCC using ab233477 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (ab233477)Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for SHC3/SHCC using ab233477 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (ab233477)Formalin-fixed, paraffin-embedded human kidney tissue stained for SHC3/SHCC using ab233477 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (ab233477)Formalin-fixed, paraffin-embedded human glioma tissue stained for SHC3/SHCC using ab233477 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (ab233477)Formalin-fixed, paraffin-embedded human liver tissue stained for SHC3/SHCC using ab233477 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-SHC3/SHCC antibody (ab233477)All lanes : Anti-SHC3/SHCC antibody (ab233477) at 3 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
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Western blot - Anti-SHC3/SHCC antibody (ab233477)Anti-SHC3/SHCC antibody (ab233477) at 2 µg/ml + Recombinant human SHC3/SHCC protein
Predicted band size: 64 kDa