Anti-SF4 antibody (ab251676)
Key features and details
- Rabbit polyclonal to SF4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SF4 antibody
See all SF4 primary antibodies -
Description
Rabbit polyclonal to SF4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SF4 aa 1-150.
Database link: Q8IWZ8 -
Positive control
- IHC-P: Human cerebral cortex tissue. ICC/IF: U-251 MG cells. WB: U-2 OS cells transfected with control siRNA. NIH/3T3 and NBT-II cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SF4 antibody (ab251676)PFA-fixed, Triton X-100 permeabilized U-251 MG (formally U-373 MG) (human brain glioma cell line) cells stained for SF4 (green) using ab251676 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF4 antibody (ab251676)Paraffin-embedded human cerebral cortex tissue stained for SF4 using ab251676 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-SF4 antibody (ab251676)All lanes : Anti-SF4 antibody (ab251676) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
Predicted band size: 72 kDa
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Western blot - Anti-SF4 antibody (ab251676)All lanes :
Lane 1 : U-2 OS cells transfected with siRNA probe #1
Lane 2 : U-2 OS cells transfected with siRNA probe #2
Lane 3 : U-2 OS cells transfected with control siRNA
Predicted band size: 72 kDa