Anti-Serotonin transporter antibody (ab172884)
Key features and details
- Rabbit polyclonal to Serotonin transporter
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-Serotonin transporter antibody
See all Serotonin transporter primary antibodies -
Description
Rabbit polyclonal to Serotonin transporter -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
-
Immunogen
Synthetic peptide corresponding to Rat Serotonin transporter aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P31652 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Serotinergic Nucleus; Rat Serotinergic Nucleus; Mouse Substantia Nigra; Rat Substantia Nigra; Rat Cerebellum.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications SheepCowHumanChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa).Target
-
Function
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. -
Tissue specificity
Expressed in platelets (at protein level). -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. -
Post-translational
modificationsGlycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616. -
Cellular localization
Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. - Information by UniProt
-
Database links
- Entrez Gene: 282365 Cow
- Entrez Gene: 6532 Human
- Entrez Gene: 15567 Mouse
- Entrez Gene: 25553 Rat
- Entrez Gene: 443491 Sheep
- Omim: 182138 Human
- SwissProt: Q9XT49 Cow
- SwissProt: P31645 Human
see all -
Alternative names
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
see all
Images
-
Western blot - Anti-Serotonin transporter antibody (ab172884)All lanes : Anti-Serotonin transporter antibody (ab172884) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Mouse Serotinergic Nucleus Tissue Lysate
Lane 2 : Rat Serotinergic Nucleus Tissue Lysate
Lane 3 : Mouse Substantia Nigra Tissue Lysate
Lane 4 : Rat Substantia Nigra Tissue Lysate
Lane 5 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 60,62 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab172884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (2)
ab172884 has been referenced in 2 publications.
- Han X et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull 139:256-262 (2018). WB . PubMed: 29524471
- Lin WH et al. Novel insights into the effect of paroxetine administration in pilocarpine-induced chronic epileptic rats. Mol Med Rep 16:8245-8252 (2017). PubMed: 28983622
Images
-
Western blot - Anti-Serotonin transporter antibody (ab172884)All lanes : Anti-Serotonin transporter antibody (ab172884) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Mouse Serotinergic Nucleus Tissue Lysate
Lane 2 : Rat Serotinergic Nucleus Tissue Lysate
Lane 3 : Mouse Substantia Nigra Tissue Lysate
Lane 4 : Rat Substantia Nigra Tissue Lysate
Lane 5 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 60,62 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab172884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
