Anti-RABGGTB antibody (ab187717)
Key features and details
- Rabbit polyclonal to RABGGTB
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RABGGTB antibody
See all RABGGTB primary antibodies -
Description
Rabbit polyclonal to RABGGTB -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human RABGGTB aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P53611 -
Positive control
- HeLa, 293T, Jurkat, Mouse TCMK-1 and Mouse NIH3T3 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RABGGTB antibody (ab187717)All lanes : Anti-RABGGTB antibody (ab187717) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse TCMK-1 cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-RABGGTB antibody (ab187717)Detection of Human RABGGTB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: ab187717 used for IP at 6 μg per reaction (Lane 2). RABGGTB was also immunoprecipitated using an other antibody (Lane 1). Lane 3 is the Control IgG IP. For blotting immunoprecipitated RABGGTB, ab187717 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds
Lane 1 : Rabbit Anti-RABGGTB at 6 µg
Lane 2 : Anti-RABGGTB antibody (ab187717) at 6 µg
Lanes 1-2 : Immunoprecipitation of RABGGTB from 293T cell lysate
Lane 3 : Immunoprecipitation of control IgG from 293T cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
