Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18747] to Serine racemase - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Serine racemase antibody [EPR18747] - BSA and Azide free
    See all Serine racemase primary antibodies

  • Description

    Rabbit monoclonal [EPR18747] to Serine racemase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250608 is the carrier-free version of ab182217. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250608 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)
    Western blot - Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)
    All lanes : Anti-Serine racemase antibody [EPR18747] (ab182217) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 34-36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 41 seconds


    This data was developed using ab182217, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with the literature; heart tissue is expected to be negative for serine racemase (PMID: 10557334).

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)
    Immunoprecipitation - Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)

    This data was developed using ab182217, the same antibody clone in a different buffer formulation.

    Serine racemase was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab182217 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182217 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg (Input). 

    Lane 2: ab182217 IP in mouse brain tissue lysate. 

    Lane 3 Rabbit monoclonal IgG (ab172730) instead of ab182217 in mouse brain tissue lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)
    Anti-Serine racemase antibody [EPR18747] - BSA and Azide free (ab250608)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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