Anti-Septin 8 antibody [EPR16099] (ab191404)
![Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228717-anti-septin-8-antibody-epr16099-western-blot.jpg "Anti-Septin 8 antibody [EPR16099] (ab191404)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16099] to Septin 8
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Septin 8 antibody [EPR16099]
See all Septin 8 primary antibodies -
Description
Rabbit monoclonal [EPR16099] to Septin 8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF RatIHC-P MouseHumanIP HumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, HeLa, HCT-116, U-87 MG, C6, PC-12 and NIH/3T3 cell lysates; Human stomach and mouse brain tissues; K562 and C6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16099 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404)All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228716-anti-septin-8-antibody-epr16099-western-blot.jpg)
Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404)All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : U-87 MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228715-anti-septin-8-antibody-epr16099-western-blot.jpg)
Western blot - Anti-Septin 8 antibody [EPR16099] (ab191404)All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228714-anti-septin-8-antibody-epr16099-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (ab191404)Immunohistochemical analysis of paraffin-embedded, Human stomach tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228713-anti-septin-8-antibody-epr16099-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] (ab191404)Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228712-anti-septin-8-antibody-epr16099-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody [EPR16099] (ab191404)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized C6 cells labeling Septin 8 with ab191404 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).
The two negative controls used anti-Septin 8 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
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![Flow Cytometry - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228711-anti-septin-8-antibody-epr16099-flow-cytometry.jpg)
Flow Cytometry - Anti-Septin 8 antibody [EPR16099] (ab191404)Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling Septin 8 with ab191404 at 1/150 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti-rabbit IgG (FITC) was used as secondary antibody at 1/150 dilution.
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![Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-228710-anti-septin-8-antibody-epr16099-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] (ab191404)Western blot analysis of Septin 8 in K562 cell lysate immunoprecipitated using ab191404 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-Septin 8 antibody [EPR16099] (ab191404)](https://www.abcam.com/ps/products/191/ab191404/Images/ab191404-9-benefits-of-recombinant-antibodies.png)
Anti-Septin 8 antibody [EPR16099] (ab191404)
