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Anti-Separase antibody (ab52158)

Anti-Separase antibody (ab52158)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Separase
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Separase antibody
    See all Separase primary antibodies
  • Description

    Rabbit polyclonal to Separase
  • Host species

    Rabbit
  • Specificity

    ab52158 detects endogenous levels of total SEPARASE protein.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic non-phosphopeptide derived from human Separase around the phosphorylation site of serine 801 (N-S-S-P-V).

  • Positive control

    • human colon carcinoma, extracts from A431 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Images

  • Western blot - Anti-Separase antibody (ab52158)
    Western blot - Anti-Separase antibody (ab52158)
    All lanes : Anti-Separase antibody (ab52158) at 1/500 dilution

    Lane 1 : extracts from A431 cells
    Lane 2 : extracts from A431 cells, with immunizing peptide

    Predicted band size: 233 kDa
    Observed band size: 233 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Separase antibody (ab52158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Separase antibody (ab52158)
    Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1:50 dilution). Left: sample untreated. Right: sample treated with immunizing peptide (negative control).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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