Anti-IP3R1 antibody (ab264281)
Key features and details
- Rabbit polyclonal to IP3R1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IP3R1 antibody
See all IP3R1 primary antibodies -
Description
Rabbit polyclonal to IP3R1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human IP3R1 aa 2708-2758. The exact sequence is proprietary.
Database link: Q14643 -
Positive control
- IHC-P: Mouse cerebellum tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IF: Mouse cerebellum tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (ab264281)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/100 dilution. Detection: Red fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstain: DAPI (blue).
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Western blot - Anti-IP3R1 antibody (ab264281)All lanes : Anti-IP3R1 antibody (ab264281) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 314 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-IP3R1 antibody (ab264281)IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264281 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264281 at 0.4 µg/ml.
Lane 1: ab264281 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (ab264281)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/200 dilution. Detection: DAB. Counterstain: IHC Hematoxylin (blue).
