Anti-CARD8 antibody (ab24186)
Key features and details
- Rabbit polyclonal to CARD8
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CARD8 antibody
See all CARD8 primary antibodies -
Description
Rabbit polyclonal to CARD8 -
Host species
Rabbit -
Specificity
ab24186 reacts with CARD8 (CARDINAL/TUCAN) protein. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to amino acids within the last 50 amino acids of the C terminus of human CARD8, GenBank accession AAG50014
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CARD8 antibody (ab24186)Lane 1 : Anti-CARD8 antibody (ab24186) at 2 µg/ml (CARD8 expression in K562 cell lysate)
Lane 2 : Anti-CARD8 antibody (ab24186) at 4 µg/ml (CARD8 expression in K562 cell lysate)
All lanes :
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
A peptide blocking study was also done (data not shown) and the only bands that disappeared were around 45kDa. The other bands are thought to be non-specific, but more testing to confirm or refute this has not been performed. -
Immunocytochemistry/ Immunofluorescence - Anti-CARD8 antibody (ab24186)ab24186 at 10µg/ml staining CARD8 in K562 cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-CARD8 antibody (ab24186) Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab24186 staining CARD8 in human monocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab24186 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-CARD8 antibody (ab24186) This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappaab24186 staining CARD8 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA = Wheat Germ Agglutinin
